MUELLER HINTON AGAR is used for cultivation of Neisseria & for determination of susceptibility of
microorganisms to antibiotics. It is formulated by Mueller and Hinton for the primary isolation of
Neisseria species. Bauer and Kirby recommended this medium for performing antibiotic susceptibility
tests using a single disc of high concentration. It has become the standard medium for antimicrobial
susceptibility testing and its performance is in accordance to Clinical and Laboratory Standard Institute
(CLSI), formerly NCCLS and complies with requirements of the WHO and FDA.